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249: Micro Minutes: CLSI M100: Beyond S, I, and R

Release Date: 10/22/2026

249: Micro Minutes: CLSI M100: Beyond S, I, and R

Let's Talk Micro

When you open CLSI M100, what are you usually looking for? Susceptibility breakpoints? But did you know there's much more information available beyond susceptible, intermediate, and resistant interpretations? In this episode of Micro Minutes, we explore useful information found throughout CLSI M100, including its tables, comments, and appendices. From intrinsic resistance and predicting susceptibility to other antimicrobials to understanding why certain agents should not be reported as susceptible, we highlight practical examples that can help clinical microbiology professionals interpret...

248: Micro Minutes: What's in a name- Bacterial Groups and Complexes

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Have you ever performed an organism identification using MALDI-TOF mass spectrometry and received a result showing multiple possible species, such as Enterobacter cloacae and Enterobacter asburiae, or Citrobacter freundii and Citrobacter werkmanii? Why does this happen? What do these organisms have in common, and why are they classified into groups or complexes? In this episode of Micro Minutes, Luis explores bacterial groups and species complexes, why they exist, and how they relate to organism identification. He discusses several familiar and lesser-known examples, including the Enterobacter...

247: Digital Biology: Data Quality in the Age of AI

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As artificial intelligence (AI) becomes increasingly integrated into biological research, the quality of the data used to develop these technologies is more important than ever. But what exactly is digital biology, and why does data quality matter? In this episode of Let's Talk Micro, Luis is joined by Jonathan Jacobs, PhD, Senior Director of Bioinformatics at ATCC, to explore digital biology and the challenges associated with biological and genomic data. Dr. Jacobs discusses the importance of metadata, common issues with publicly available genomic databases, and why connecting digital...

246: Inside ATCC: From Biological Materials to the Bench

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Many microbiology professionals are familiar with ATCC strains used for quality control, validation, and verification—but what happens before those biological materials reach the laboratory? ATCC, the American Type Culture Collection, maintains biological materials used by scientists and laboratories around the world. In this episode of Let’s Talk Micro, Luis is joined by Dr. Shahin Ali, Senior Scientist at ATCC, to take a closer look at the work behind biological reference materials. Dr. Ali discusses how ATCC identifies and acquires microorganisms, what characteristics are considered...

World Rabies Day: Revisiting Rabies with Dr. Rodney Rohde

Let's Talk Micro

Originally published on September 28, 2022, this episode returns to the Let’s Talk Micro feed in recognition of World Rabies Day. Dr. Rodney Rohde joins the podcast to discuss rabies and his extensive experience working with the virus in public health. We start with the basics: What is rabies? How is it transmitted? What are the signs and symptoms? From there, we discuss potential exposures, post-exposure prophylaxis, vaccination, and ways to prevent rabies. Dr. Rohde also shares his experience working in rabies diagnostics and molecular epidemiology, as well as his involvement with wildlife...

245: Antimicrobials Conference: The Next Chapter for MAD-ID and SIDP

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MAD-ID (Making a Difference in Infectious Diseases) and the Society of Infectious Diseases Pharmacists (SIDP) have joined forces to create a new annual meeting focused on antimicrobial therapeutics, stewardship, infectious diseases, and more. In this episode of Let's Talk Micro, Luis is joined by Dr. Erin McCreary and Dr. Mike Rybak to discuss the history of MAD-ID and SIDP, what brought the two organizations together, and highlights from their 2026 meeting. They also discuss sessions relevant to clinical microbiology, practical approaches to applying new science, opportunities for networking...

244: Group A Strep: Breaking Down the New IDSA Guidelines

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Group A Streptococcus (GAS) is a common cause of pharyngitis, but deciding who should be tested and which testing method to use isn’t always straightforward. In 2025, the Infectious Diseases Society of America (IDSA) released updated guidelines on Group A streptococcal pharyngitis, bringing renewed attention to diagnostic stewardship and the use of clinical scoring systems to help guide testing decisions. In this episode of Let’s Talk Micro, Dr. Christopher Doern returns to the podcast to break down the updated IDSA guidelines and what they may mean for clinical practice. We discuss...

243: When AI Meets Medicine

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Artificial intelligence is changing how healthcare professionals access and use clinical information. But what does that look like at the point of care? In this episode of Let’s Talk Micro, Luis is joined by Dr. Declan Kelly, physician, software engineer, and founder of Eolas Medical, to discuss the role of AI in clinical decision support. Dr. Kelly shares how challenges accessing institutional guidelines and clinical knowledge led to the development of Eolas Medical and how AI can help healthcare professionals find the information they need when making clinical decisions. The conversation...

242: Microbiology & Infection Prevention: Partners in Crime

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Let’s Talk Micro is back for a new season! In this season premiere, Luis is joined by Dr. Megan Amerson-Brown to discuss the important connection between clinical microbiology and infection prevention. Dr. Amerson-Brown shares her career journey from the microbiology bench to infection prevention and ultimately laboratory leadership. She discusses how microbiologists contribute to infection prevention through surveillance, diagnostic stewardship, outbreak investigations, and collaboration with infection prevention teams. The conversation also explores how laboratorians can become more...

241: What Can Whole Genome Sequencing Realistically Tell Us About Resistance?

Let's Talk Micro

Recorded live at ASM Microbe 2026 in Washington, DC, this special episode marks the first live recording of Let's Talk Micro. Joining me are co-host Dr. Andrea Prinzi and guest Dr. Nathan Ledeboer for a discussion on the evolving role of whole genome sequencing (WGS) in clinical microbiology and antimicrobial resistance. Can whole genome sequencing predict antimicrobial susceptibility? What are its current limitations? How close are we to incorporating WGS into routine clinical microbiology laboratories? Throughout the conversation, we explore the opportunities and challenges of implementing...

More Episodes

In this episode of Micro Minutes, we explore useful information found throughout CLSI M100, including its tables, comments, and appendices. From intrinsic resistance and predicting susceptibility to other antimicrobials to understanding why certain agents should not be reported as susceptible, we highlight practical examples that can help clinical microbiology professionals interpret antimicrobial susceptibility results.

Whether you're a student or an experienced laboratory professional, join us as we take a closer look at CLSI M100 and discover what's beyond S, I, and R.

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